Figure 5. G. lucidum-derived subfraction 10K-2 attenuates FIB-1 expression, extends lifespan, and induces autophagy in C. elegans. (A) Effects of G. lucidum (GL) sub-fractions on FIB-1::GFP intensity. Synchronized L4 larvae of C. elegans SJL1 were treated with water, GL sub-fractions, or rapamycin (Rapa; 400 μM) for 3 days. FIB-1::GFP was monitored by fluorescence microscopy. (B) Lifespan assay of C. elegans treated with GL sub-fractions. Synchronized C. elegans SJL1 larvae were treated with water, GL subfractions 10K-1 (1 mg/plate) or 10K-2 (2 mg/plate), or rapamycin (400 μM). Representative survival curves are shown (see also Supplementary Table 1). (C) GL sub-fraction 10K-2 induces autophagy in C. elegans. DA2123 nematodes were treated with the sub-fractions for 3 days, and GFP::LGG-1 levels were quantified by fluorescence microscopy. Numbers of GFP puncta were counted in 50 seam cells. Data represent means ± standard deviation. Statistical analysis was done using Student’s t test. *p<0.05; ***p<0.001.