Research Paper Volume 13, Issue 11 pp 15078—15099

Mechanistic insights into the anti-depressant effect of emodin: an integrated systems pharmacology study and experimental validation

EMO improves CUMS induced depression-related behaviors. (A) Diagram of the experimental workflow. Twenty-four rats were randomly chosen as the control (CON) group, and 64 rats were exposed to 5 weeks of CUMS. On day 36, all rats underwent SPT to evaluate their status. Depressive-tendency rats (DET, n = 30) were defined as those with a more than a 20% decrease in sucrose water intake, and then divided into two groups: the DET+vehicle (VEH) group and DET+EMO (80 mg/kg/day) group (n = 15/group). After two weeks of EMO treatment, the animals completed the SPT, OFT and FST. (B) Percentage of sucrose water consumed in the SPT at day 52. The numbers of zone crossings in the OFT (C) and the immobility times in the FST (D) were recorded. (E) The body weight was measured at day 51. Data were expressed as the means ± SEM. *** p p p

Figure 1. EMO improves CUMS induced depression-related behaviors. (A) Diagram of the experimental workflow. Twenty-four rats were randomly chosen as the control (CON) group, and 64 rats were exposed to 5 weeks of CUMS. On day 36, all rats underwent SPT to evaluate their status. Depressive-tendency rats (DET, n = 30) were defined as those with a more than a 20% decrease in sucrose water intake, and then divided into two groups: the DET+vehicle (VEH) group and DET+EMO (80 mg/kg/day) group (n = 15/group). After two weeks of EMO treatment, the animals completed the SPT, OFT and FST. (B) Percentage of sucrose water consumed in the SPT at day 52. The numbers of zone crossings in the OFT (C) and the immobility times in the FST (D) were recorded. (E) The body weight was measured at day 51. Data were expressed as the means ± SEM. *** p < 0.001 DET+VEH vs CON+VEH. # p < 0.05, ### p < 0.001 DET+EMO vs DET+VEH.