Research Paper Volume 13, Issue 11 pp 15078—15099

Mechanistic insights into the anti-depressant effect of emodin: an integrated systems pharmacology study and experimental validation

EMO treatment significantly reduced CUMS induced microglial activation and neuroinflammation. (A) Microglia in the PFC were identified by Iba1 immunohistochemical staining (bar = 10 μm). (B) The solidities of microglia were calculated to evaluate the activation of microglia (n = 3, 8-9 slices/group). Levels of IL-1β (C) and TNF-α (D) in the prefrontal cortex (PFC) were detected by ELISA (pg/mg protein, n = 6). The level of SOD activity (E) in the PFC was measured by ELISA (U/mg protein, n = 6). Data were expressed as the means ± SEM. *** p p

Figure 8. EMO treatment significantly reduced CUMS induced microglial activation and neuroinflammation. (A) Microglia in the PFC were identified by Iba1 immunohistochemical staining (bar = 10 μm). (B) The solidities of microglia were calculated to evaluate the activation of microglia (n = 3, 8-9 slices/group). Levels of IL-1β (C) and TNF-α (D) in the prefrontal cortex (PFC) were detected by ELISA (pg/mg protein, n = 6). The level of SOD activity (E) in the PFC was measured by ELISA (U/mg protein, n = 6). Data were expressed as the means ± SEM. *** p < 0.001 DET+VEH vs CON+VEH. ### p < 0.001 DET+EMO vs DET+VEH.