Figure 2. CXCR2 is overexpressed and modulates the expression of CXCL2 and CXCL8 in ACRP cells and CXCR2 KD. Transcript expression of CXCR2, CXCL2 and CXCL8 was investigated by qRT-PCR, following the protocol described in Material and Methods session, in both wild-type and CXCR2 KD (siRNA CXCR2, 10μM) A2780 and ACRP OC cells. (A) ACRP expressed 2.3-fold more CXCR2 than A2780 (#p=0.0342). (B) CXCR2 KD lead to lower expression of CXCR2 in both cell lines, however 1.3-fold less in ACRP (***p=0.001) than in A2780 (*0.0246). (C) CXCL2 expression was 2.5-fold lower in ACRP CXCR2 KD than in ACRP NC (+p=0.0362). (D) CXCL8 expression decreased by 6.5-fold comparing ACRP CXCR2 KD to ACRP NC (**p=0.0025). Differential gene expression was presented as relative expression of each gene of interest compared to control, after normalization by the expression of the housekeeping gene GAPDH and calculated by the 2-ΔΔCt method. Data were analyzed by unpaired t-Student test (p<0.05). N=3.