Research Paper Volume 13, Issue 10 pp 13405—13420

Inhibition of CXCR2 plays a pivotal role in re-sensitizing ovarian cancer to cisplatin treatment

CXCR2 promotes in vivo OC tumor growth (TG), angiogenesis and tumor invasion.In vivo investigation of the role of CXCR2 on tumor growth, invasion and angiogenesis in OC was assessed by chicken embryo chorioallantoic membrane (CAM) method. (A) The number of new vessels formation with diameter lower than 20μm growing radially towards the ring area was counted in a blind fashion manner. No differences were noted between the lineages under the referred experimental condition. (B) Tumor size was measured at E10 egg inoculation with A2780 and ACRP OC cells, as: i) NC; ii) siRNA CXCR2 KD cells. Statistic significant difference were not observed probably due to number of replicates performed. However, when compared to A2780, TG was higher in ACRP than in A2780 cells. Moreover, CXCR2 KD prevented TG in later. The overall mortality rate for embryos/eggs was as expected (~10%). (C) The figure is also of a distinct invasion patterns between NC and CXCR2 KD ACRP and A2780 cells.

Figure 7. CXCR2 promotes in vivo OC tumor growth (TG), angiogenesis and tumor invasion.In vivo investigation of the role of CXCR2 on tumor growth, invasion and angiogenesis in OC was assessed by chicken embryo chorioallantoic membrane (CAM) method. (A) The number of new vessels formation with diameter lower than 20μm growing radially towards the ring area was counted in a blind fashion manner. No differences were noted between the lineages under the referred experimental condition. (B) Tumor size was measured at E10 egg inoculation with A2780 and ACRP OC cells, as: i) NC; ii) siRNA CXCR2 KD cells. Statistic significant difference were not observed probably due to number of replicates performed. However, when compared to A2780, TG was higher in ACRP than in A2780 cells. Moreover, CXCR2 KD prevented TG in later. The overall mortality rate for embryos/eggs was as expected (~10%). (C) The figure is also of a distinct invasion patterns between NC and CXCR2 KD ACRP and A2780 cells.