Research Paper Volume 13, Issue 11 pp 15193—15213

TRDMT1 participates in the DNA damage repair of granulosa cells in premature ovarian failure

Oxidative stress induces DNA damage and apoptosis in GCs. (G) H2O2+KGN were harvested at the indicated time points for γ-H2AX staining. Positive cells in each group and the frequency of cells with foci were counted. Scale bar: 10μm. (H) KGN cells transfected with GFP-TRDMT1 (WT, G155V, and E63K mutant) were stimulated with H2O2, and their cell localization was observed. Scale bar: 50μm.Comparison of the nucleus/cytoplasm ratio between the two groups. *P

Figure 3. Oxidative stress induces DNA damage and apoptosis in GCs. (G) H2O2+KGN were harvested at the indicated time points for γ-H2AX staining. Positive cells in each group and the frequency of cells with foci were counted. Scale bar: 10μm. (H) KGN cells transfected with GFP-TRDMT1 (WT, G155V, and E63K mutant) were stimulated with H2O2, and their cell localization was observed. Scale bar: 50μm.Comparison of the nucleus/cytoplasm ratio between the two groups. *P<0.05, **p<0.01, and ***p<0.001. Statistical significance was determined using two-tailed t-tests for two groups and ANOVA for multiple comparisons. All values are means ± SD.