Research Paper Advance Articles

Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer’s disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway

BM-MSC-EVs carry miR-29c-3p into AD hippocampal neurons. (A) RT-qPCR was used to detect the expression of miR-29c-3p in BM-MSCs and their EVs; (B) RT-qPCR was used to detect the expression of miR-29c-3p in cerebral tissues of rats in each group; (C) RT-qPCR was used to detect the expression of miR-29c-3p in hippocampal neurons of each group; (D) AD neurons treated with PKH-26-labeled EVs were observed under a laser scanning confocal microscopy. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. ***p

Figure 3. BM-MSC-EVs carry miR-29c-3p into AD hippocampal neurons. (A) RT-qPCR was used to detect the expression of miR-29c-3p in BM-MSCs and their EVs; (B) RT-qPCR was used to detect the expression of miR-29c-3p in cerebral tissues of rats in each group; (C) RT-qPCR was used to detect the expression of miR-29c-3p in hippocampal neurons of each group; (D) AD neurons treated with PKH-26-labeled EVs were observed under a laser scanning confocal microscopy. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. ***p < 0.001.