Figure 4. miR-29c-3p knockdown reduces the therapeutic effects of BM-MSC-EVs on AD hippocampal neurons. BM-MSCs were transfected with miR-29c-3p inhibitor with inhibitor NC as control. Then EVs were isolated (EVs-inhibitor/ EVs-inhibitor NC) to treat AD neurons. (A) RT-qPCR was used to detect the effect of miR-29c-3p inhibitor on the expression of miR-29c-3p in each group; (B) Immunofluorescence assay was used to detect the content of Aβ in hippocampal neurons of each group; (C) ELISA was used to detect the level of Aβ1-42 in AD hippocampal neurons; (D) MTT assay was used to detect the viability of AD hippocampal neurons; (E) Flow cytometry was used to detect the apoptosis rate of AD hippocampal neurons. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Comparisons between two groups were analyzed using independent sample t-test; comparisons among multiple groups were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. **p < 0.01; **p < 0.001.