Research Paper Volume 13, Issue 11 pp 15285—15306

Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer’s disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway

miR-29c-3p targets BACE1 expression in AD. (A) The binding sites of miR-145-3p and BACE1 were predicted through database (<a href="http://starbase.sysu.edu.cn/agoClipRNA.php?source=mRNA" target="

Figure 5. miR-29c-3p targets BACE1 expression in AD. (A) The binding sites of miR-145-3p and BACE1 were predicted through database (http://starbase.sysu.edu.cn/agoClipRNA.php?source=mRNA); (B) Dual-luciferase reporter gene assay was used to verify the binding relationship between miR-145-3p and BACE1; (C) RT-qPCR was used to detect the mRNA expression of BACE1 in rat cerebral tissues and neurons in each group; (D) WB was used to detect the protein level of BACE1 in rat cerebral tissues and neurons in each group. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Data were analyzed using one-way ANOVA followed by Sidak’s multiple comparisons test. **p < 0.01; **p < 0.001.