Figure 8. Inhibition of the Wnt/β-catenin pathway impairs the therapeutic effects of BM-MSC-EVs on AD. The Wnt/β-catenin pathway inhibitor DKK1 was added to BM-MSC-EVs-treated AD neurons, with the addition of PBS as control. (A) The content of Aβ in AD hippocampal neurons was detected using immunofluorescence assay; (B) Level of Aβ1-42 in AD hippocampal neurons was detected using ELISA; (C) MTT assay was used to detect the viability of AD hippocampal neurons; (D) Flow cytometry was used to detect the apoptosis rate of AD hippocampal neurons. The experiment was repeated three times, and the data were expressed as mean ± standard deviation. Data among groups were compared using one-way ANOVA, followed by Tukey’s multiple comparisons test was used. **p < 0.01; ***p < 0.001.