Research Paper Volume 13, Issue 11 pp 15353—15365

Alisol A 24-acetate protects against brain microvascular endothelial cells injury through inhibiting miR-92a-3p/tight junctions axis

Messenger ribonucleic acid (mRNA) expression of tight junctions (TJs) in OGD-induced bEnd.3 cells. (A–C) Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was applied to detect the mRNA expression of zonula occludens (ZO-1), claudin-5, and occludin. The quantity was calculated. The housekeeping gene used to normalize the mRNA expression is GAPDH mRNA (OGD-L: 1.9 umol/L Alisol A 24-acetate; OGD-M: 19 umol/L Alisol A 24-acetate; OGD-H: 38 umol/L Alisol A 24-acetate). Results are described as means ± SD (n = 6) (**P ##P

Figure 3. Messenger ribonucleic acid (mRNA) expression of tight junctions (TJs) in OGD-induced bEnd.3 cells. (AC) Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was applied to detect the mRNA expression of zonula occludens (ZO-1), claudin-5, and occludin. The quantity was calculated. The housekeeping gene used to normalize the mRNA expression is GAPDH mRNA (OGD-L: 1.9 umol/L Alisol A 24-acetate; OGD-M: 19 umol/L Alisol A 24-acetate; OGD-H: 38 umol/L Alisol A 24-acetate). Results are described as means ± SD (n = 6) (**P < 0.01 vs. control group, ##P < 0.01 vs. OGD group).