Research Paper Volume 13, Issue 11 pp 15353—15365

Alisol A 24-acetate protects against brain microvascular endothelial cells injury through inhibiting miR-92a-3p/tight junctions axis

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Figure 5. Expressions of miR-92a-3p in OGD-induced bEnd.3 cells and transfection of miR-92a-3p mimics and inhibitor. (A) Expressions of miR-92a-1-5p and miR-92a-3p in cells were detected by qPCR (miNC: mimics negative control; inNC: inhibitor negative control; mi92: miR-92a-3p mimics; mi92+M: miR-92a-3p mimics +19 umol/L Alisol A 24-acetate; in92: miR-92a-3p inhibitor). Results are presented as means ± SD (n = 6) (**P < 0.01 vs. control group; ##P < 0.01 vs. OGD group). (B, C) MiR-92a-3p expression of transfection with miR-92a-3p mimics and inhibitor assessed by qRT-PCR. (D) Cell viability of miR-92a-3p transfection was calculated. (E) LDH levels were calculated. Results are described as the mean ± SD (n = 6) (**P < 0.01 vs. control group; ##P < 0.01 vs. OGD group; @@P < 0.01 vs. mi92a group).