Research Paper Volume 13, Issue 11 pp 15353—15365

Alisol A 24-acetate protects against brain microvascular endothelial cells injury through inhibiting miR-92a-3p/tight junctions axis

MiR-92a-3p directly targets ZO-1 and occludin genes. (A) MiR-92a-3p directly binds to ZO-1. Different types of luciferase reporter vectors were constructed: wild-type ZO-1 3’UTR (ZO-1 wild-type (WT)) and mutant-type ZO-1 3’UTR (ZO-1 MT). The above vectors were co-transfected into HEK-293 cells with miR-92a-3p mimics or mimics NC and examined for luciferase activity. (B) MiR-92a-3p directly binds to occludin. Different types of luciferase reporter vectors were constructed: wild-type occludin 3’UTR (Ocln WT) and mutant-type occludin 3’UTR (Ocln MT). The above vectors were co-transfected into HEK-293 cells with miR-92a-3p mimics or mimics NC and examined for luciferase activity. Each bar represents the mean ± SD of three independent experiments (*P

Figure 7. MiR-92a-3p directly targets ZO-1 and occludin genes. (A) MiR-92a-3p directly binds to ZO-1. Different types of luciferase reporter vectors were constructed: wild-type ZO-1 3’UTR (ZO-1 wild-type (WT)) and mutant-type ZO-1 3’UTR (ZO-1 MT). The above vectors were co-transfected into HEK-293 cells with miR-92a-3p mimics or mimics NC and examined for luciferase activity. (B) MiR-92a-3p directly binds to occludin. Different types of luciferase reporter vectors were constructed: wild-type occludin 3’UTR (Ocln WT) and mutant-type occludin 3’UTR (Ocln MT). The above vectors were co-transfected into HEK-293 cells with miR-92a-3p mimics or mimics NC and examined for luciferase activity. Each bar represents the mean ± SD of three independent experiments (*P < 0.05).