Figure 3. BCRT1 enhanced osteosarcoma cell cycle and proliferation. (A) The expression of BCRT1 in four osteosarcoma cell lines (MG-63, HOS, SAOS-2 and U2OS) and hFOB was determined by RT-qPCR analysis. (B) The level of BCRT1 was detected using RT-qPCR analysis. (C) Ectopic expression of BCRT1 increased ki-67 expression in MG-63 cell. (D) The level of cyclin D1 was determined using RT-qPCR method. (E) The level of CKD2 was determined using RT-qPCR method. (F) Elevated expression of BCRT1 promoted cell growth in MG-63 cell. (G) Overexpression of BCRT1 increased cell cycle in MG-63 cell. *p<0.05, **p<0.01 and ***p<0.001.