Figure 4. BCRT1 induced EMT progression and inflammatory mediators secretion in osteosarcoma cell. (A) Overexpression of BCRT1 inhibited E-cadherin expression in MG-63 cell. (B) The expression of N-cadherin was detected by qRT-PCR assay. (C) The level of vimentin was measured using qRT-PCR assay. (D) Elevated expression of BCRT1 induced IL-1β secretion. (E) The level of TGFβ was determined by ELISA assay. (F) The level of IL-6 was measured by ELISA assay. **p<0.01 and ***p<0.001.