Research Paper Volume 13, Issue 11 pp 15501—15510

LncRNA BCRT1 facilitates osteosarcoma progression via regulating miR-1303/FGF7 axis

BCRT1 regulated miR-1303/FGF7 expression in osteosarcoma cell. (A) BCRT1 may sponge miR-1303 expression using starbase bioinformatics algorithm. (B) The expression of miR-1303 was determined by qRT-PCR assay. (C) miR-1303 overexpression suppressed luciferase value of wild BCRT1 3’UTR but not the mutant BCRT1 3’UTR in MG-63 cell. (D) Elevated expression of BCRT1 decreased miR-1303 expression in MG-63 cell. (E) FGF7 may one target gene of miR-1303 using targetscan bioinformatics algorithm. (F) Luciferase reporter analysis noted that miR-1303 overexpression suppresses luciferase value of wild FGF7 3’UTR but not the mutant FGF7 3’UTR in MG-63 cell. (G) Ectopic expression of miR-1303 suppressed FGF7 expression in MG-63 cell. (H) Elevated expression of BCRT1 enhanced FGF7 expression in MG-63 cell. **p

Figure 5. BCRT1 regulated miR-1303/FGF7 expression in osteosarcoma cell. (A) BCRT1 may sponge miR-1303 expression using starbase bioinformatics algorithm. (B) The expression of miR-1303 was determined by qRT-PCR assay. (C) miR-1303 overexpression suppressed luciferase value of wild BCRT1 3’UTR but not the mutant BCRT1 3’UTR in MG-63 cell. (D) Elevated expression of BCRT1 decreased miR-1303 expression in MG-63 cell. (E) FGF7 may one target gene of miR-1303 using targetscan bioinformatics algorithm. (F) Luciferase reporter analysis noted that miR-1303 overexpression suppresses luciferase value of wild FGF7 3’UTR but not the mutant FGF7 3’UTR in MG-63 cell. (G) Ectopic expression of miR-1303 suppressed FGF7 expression in MG-63 cell. (H) Elevated expression of BCRT1 enhanced FGF7 expression in MG-63 cell. **p<0.01 and ***p<0.001.