Priority Research Paper Volume 13, Issue 10 pp 13380—13392

Cdkn1a transcript variant 2 is a marker of aging and cellular senescence

Cdkn1a variant 1 is circadian regulated, whereas variant 2 rises with senescence in vivo. (A) Cdkn1a variants expression throughout the light cycle (6 am to 6 pm) in the liver, (B) white adipose tissue and (C) kidney of 6 week-old male mice. (D–F) A cohort of 6 week-old mice were treated with doxorubicin or vehicle (n = 9-10 for either males or females, n = 9-10 for either vehicle or doxorubicin) and Cdkn1a variant levels were analyzed 6 weeks later. Results are shown for (D) liver, (E) adipose tissue and (F) kidney. (G–I) A cohort of 18 to 22 month-old mice were treated with ABT-263 or vehicle. Pooled results are shown for (G) liver, (H) adipose tissue and (I) kidney. 1-way ANOVA and Tukey post-tests were applied. * p

Figure 3. Cdkn1a variant 1 is circadian regulated, whereas variant 2 rises with senescence in vivo. (A) Cdkn1a variants expression throughout the light cycle (6 am to 6 pm) in the liver, (B) white adipose tissue and (C) kidney of 6 week-old male mice. (DF) A cohort of 6 week-old mice were treated with doxorubicin or vehicle (n = 9-10 for either males or females, n = 9-10 for either vehicle or doxorubicin) and Cdkn1a variant levels were analyzed 6 weeks later. Results are shown for (D) liver, (E) adipose tissue and (F) kidney. (GI) A cohort of 18 to 22 month-old mice were treated with ABT-263 or vehicle. Pooled results are shown for (G) liver, (H) adipose tissue and (I) kidney. 1-way ANOVA and Tukey post-tests were applied. * p < 0.05, ** p < 0.01, *** p < 0.001, ns = not significant.