Figure 3. PU.1 was positive associated with expression of S100A8/9 and identified as a transcription factor of them. (A) Transcriptionally activity of PU.1 on (A) S100A8 and (B) S100A9 gene promoters was detected by luciferase reporter gene assay. Pearson correlation coefficient assay was used to evaluate the correlations of (C) S100A8 and S100A9 expression levels, (D) S100A8 and PU.1 expression levels, and (E) S100A9 and PU.1 expression levels. ChIP-qPCR assay was used to validate the binding of PU.1 with (F) S100A8 promoter and (G) S100A9 promoter. **P < 0.01.