Research Paper Volume 13, Issue 11 pp 15523—15537

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

PU.1 was positive associated with expression of S100A8/9 and identified as a transcription factor of them. (A) Transcriptionally activity of PU.1 on (A) S100A8 and (B) S100A9 gene promoters was detected by luciferase reporter gene assay. Pearson correlation coefficient assay was used to evaluate the correlations of (C) S100A8 and S100A9 expression levels, (D) S100A8 and PU.1 expression levels, and (E) S100A9 and PU.1 expression levels. ChIP-qPCR assay was used to validate the binding of PU.1 with (F) S100A8 promoter and (G) S100A9 promoter. **P

Figure 3. PU.1 was positive associated with expression of S100A8/9 and identified as a transcription factor of them. (A) Transcriptionally activity of PU.1 on (A) S100A8 and (B) S100A9 gene promoters was detected by luciferase reporter gene assay. Pearson correlation coefficient assay was used to evaluate the correlations of (C) S100A8 and S100A9 expression levels, (D) S100A8 and PU.1 expression levels, and (E) S100A9 and PU.1 expression levels. ChIP-qPCR assay was used to validate the binding of PU.1 with (F) S100A8 promoter and (G) S100A9 promoter. **P < 0.01.