Figure 4. PU.1 was upregulated in burned skin and promoted expression of S100A8/A9. (A) Representative blots for PU.1 in burned and matched normal skin tissues from burn patients. (B) Comparison of PU.1 in burned and matched normal skin tissues of 38 patients. Primary keratinocytes were treated with PU.1 adenoviral expression vector at amounts of 0.1, 0.5 and 1 μg. Empty adenoviral vector was applied as a negative control. After transfection for 48 h, expression levels of (C) PU.1 protein and (D) EMT marker proteins in thermal-stimulated keratinocytes at different time points. *P < 0.05 vs. 0 h or Vector, #P < 0.05 vs. 6 h or 0.5 μg/mL.