Research Paper Volume 13, Issue 11 pp 15523—15537

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

PU.1 promoted cell proliferation, invasion, and expression of EMT in human epidermal keratinocytes in vitro. Primary keratinocytes were treated with PU.1 adenoviral expression vector at amounts of 0.1, 0.5 and 1 μg. Empty adenoviral vector was applied as a negative control. After transfection for 48 h, (A) cell number, (B) cell invasion, (C) expression levels of EMT marker proteins, and (D) nuclear translocation of p-Snail were respectively detected with automatic cell counter, Transwell migration assay Western blotting, and immunofluorescence assay. *P #P $P

Figure 5. PU.1 promoted cell proliferation, invasion, and expression of EMT in human epidermal keratinocytes in vitro. Primary keratinocytes were treated with PU.1 adenoviral expression vector at amounts of 0.1, 0.5 and 1 μg. Empty adenoviral vector was applied as a negative control. After transfection for 48 h, (A) cell number, (B) cell invasion, (C) expression levels of EMT marker proteins, and (D) nuclear translocation of p-Snail were respectively detected with automatic cell counter, Transwell migration assay Western blotting, and immunofluorescence assay. *P < 0.05 vs. Vector, #P < 0.05 vs. 0.1 μg/mL, $P < 0.05 vs. 0.5 μg/mL.