Figure 5. PU.1 promoted cell proliferation, invasion, and expression of EMT in human epidermal keratinocytes in vitro. Primary keratinocytes were treated with PU.1 adenoviral expression vector at amounts of 0.1, 0.5 and 1 μg. Empty adenoviral vector was applied as a negative control. After transfection for 48 h, (A) cell number, (B) cell invasion, (C) expression levels of EMT marker proteins, and (D) nuclear translocation of p-Snail were respectively detected with automatic cell counter, Transwell migration assay Western blotting, and immunofluorescence assay. *P < 0.05 vs. Vector, #P < 0.05 vs. 0.1 μg/mL, $P < 0.05 vs. 0.5 μg/mL.