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Research Paper Volume 13, Issue 11 pp 15538—15547
miR-34a inhibits melanoma growth by targeting ZEB1
Figure 3. miR-34a inhibits proliferation and migration of melanoma cells by targeting ZEB1. (A) MTT cell proliferation assay in A375 cells with increased and suppressed miR-34a expression, and transfected with ZEB1 or ZEB1 siRNA (mean ± SEM; ** P< 0.05 vs. control, ##P< 0.05 vs. miR-34a group). (B) Transwell migration assay in A375 cells overexpressing miR-34a and transfected with ZEB1 (mean ± SEM; ** P< 0.05 vs. control, ##P< 0.05 vs. miR-34a group). (C) Transwell migration assay of A375 cells with downregulated miR-34a and transfected with si-ZEB1 (mean ± SEM. ** P< 0.05 vs. control, ##P< 0.05 vs. miR-34a inhibitor group. (D) Western blotting and real-time PCR analysis of miR-34a and ZEB1 in A375 cells transfected with ZEB1 and overexpressing miR-34a (mean ± SEM; ** P< 0.05 vs. control, ##P< 0.05 vs. miR-34a group). (E) Western blotting and real-time PCR analysis of miR-34a and ZEB1 in A375 cells with downregulated miR-34a and transfected with si-ZEB1 (mean ± SEM; ** P< 0.05 vs. control, ##P< 0.05 vs. miR-34a inhibitor group).