Research Paper Volume 13, Issue 11 pp 15569—15579

Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity

Effects of REST on IEGs. (A) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. (B) 10 μM Aβ decreased ARC mRNA expression. n = 3. (C) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. (D) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. *p **p ***p

Figure 4. Effects of REST on IEGs. (A) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. (B) 10 μM Aβ decreased ARC mRNA expression. n = 3. (C) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. (D) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 vs the corresponding control group.