Figure 4. Effects of REST on IEGs. (A) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. (B) 10 μM Aβ decreased ARC mRNA expression. n = 3. (C) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. (D) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 vs the corresponding control group.