Research Paper Volume 13, Issue 11 pp 14651—14674

Hippocampal transcriptome profiling reveals common disease pathways in chronic hypoperfusion and aging

Differentially expressed genes in young hippocampus following chronic hypoperfusion. (A) Hierarchical clustering analysis of differentially expressed mRNA transcripts following 7 and 30 days BCAS compared to Sham animals. Upregulated genes in red and downregulated genes in blue. The color scale represents the log10 (average FPKM+1) value. (B) Volcano plots of differentially expressed genes analyzed in young 7 day and young 30 day BCAS animals compared to Sham animals. The threshold of differential expression is q value C) Top 50 upregulated genes in young 7 day and young 30 day BCAS animals compared to Sham animals. (D) Venn diagram reflects the distribution of differentially expressed genes from 7 day and 30 day BCAS and Sham comparisons.

Figure 3. Differentially expressed genes in young hippocampus following chronic hypoperfusion. (A) Hierarchical clustering analysis of differentially expressed mRNA transcripts following 7 and 30 days BCAS compared to Sham animals. Upregulated genes in red and downregulated genes in blue. The color scale represents the log10 (average FPKM+1) value. (B) Volcano plots of differentially expressed genes analyzed in young 7 day and young 30 day BCAS animals compared to Sham animals. The threshold of differential expression is q value < 0.05. The horizontal axis is the log2 fold change of transcripts. The vertical axis is statistical significance scaled as -log 10 q-value. Each dot represents an individual transcript (blue: no significant difference; red: upregulated expression of gene; green: downregulated expression of gene). (C) Top 50 upregulated genes in young 7 day and young 30 day BCAS animals compared to Sham animals. (D) Venn diagram reflects the distribution of differentially expressed genes from 7 day and 30 day BCAS and Sham comparisons.