Research Paper Volume 13, Issue 11 pp 14651—14674

Hippocampal transcriptome profiling reveals common disease pathways in chronic hypoperfusion and aging

Comparative analyses of differentially expressed genes between young BCAS hippocampus and old Sham. (A) Hierarchical clustering analysis of differentially expressed genes following young 30 days BCAS and old Sham. Upregulated genes in red and down-regulated genes in blue. The color scale represents the log10 (average FPKM+1) value. (B) Differentially expressed mRNA transcripts analyzed between young 30 day BCAS and old Sham animals. The threshold of differential expression is q value C) The selection of significantly enriched down-regulated GO terms in both young 30 day BCAS and old Sham animals (q D) Venn diagram reflects the distribution of differentially upregulated and downregulated genes from young 30-day BCAS and old Sham comparisons. (E) Venn diagram reflects the distribution of differentially down-regulated mRNA transcripts from young 30-day BCAS and old Sham comparisons. (F) Common upregulated (red) and downregulated (blue) mitochondrial genes in both young 30-day BCAS and old Sham hippocampus. (G) Common and unique down-regulated protein chaperons in young 30 day BCAS and old Sham groups.

Figure 4. Comparative analyses of differentially expressed genes between young BCAS hippocampus and old Sham. (A) Hierarchical clustering analysis of differentially expressed genes following young 30 days BCAS and old Sham. Upregulated genes in red and down-regulated genes in blue. The color scale represents the log10 (average FPKM+1) value. (B) Differentially expressed mRNA transcripts analyzed between young 30 day BCAS and old Sham animals. The threshold of differential expression is q value < 0.05. The horizontal axis is the log2 fold change of transcripts. The vertical axis is statistical significance scaled as -log 10 q-value. Each dot represents an individual transcript (blue: no significant difference; red: upregulated transcript; green: down-regulated transcript). (C) The selection of significantly enriched down-regulated GO terms in both young 30 day BCAS and old Sham animals (q < 0.05). The horizontal axis represents the -log10 scale for q-value of each GO term. (D) Venn diagram reflects the distribution of differentially upregulated and downregulated genes from young 30-day BCAS and old Sham comparisons. (E) Venn diagram reflects the distribution of differentially down-regulated mRNA transcripts from young 30-day BCAS and old Sham comparisons. (F) Common upregulated (red) and downregulated (blue) mitochondrial genes in both young 30-day BCAS and old Sham hippocampus. (G) Common and unique down-regulated protein chaperons in young 30 day BCAS and old Sham groups.