Figure 4. Immunoblotting analysis of hippocampal protein levels in control and polypharmacy fed mice. Hippocampal tissue lysates of control and polypharmacy mice were analyzed by western blotting experiments. Representative immunoblots and quantification of: NMDAR1, phospho-NMDAR2A and PSD95 proteins (A, B), phospho- and total-CREB, and ratio (C, D), phospho- and total-CAMKII, and ratio (E, F), TrkB and pro-BDNF proteins (G, H). *p<0.05, Mann-Whitney (D, H) or t-Student test (F). Total protein levels were normalized with respect to α tubulin. Ctrl= control, Poly= polypharmacy, n= 10 animals per group. All data are presented as mean ± SEM.