Priority Research Paper Volume 13, Issue 11 pp 14557—14570

GRSF1 deficiency in skeletal muscle reduces endurance in aged mice

Microarray analysis of RNAs differentially expressed in skeletal muscle from Grsf1KO vs WT mice. (A) Volcano plot showing differentially expressed RNAs (including both mRNAs and long noncoding RNAs detected by probes in Agillent microarrays) in the Grsf1cKO vs WT RF muscles; n=3 mice for each genotype. (B) List of Top 25 RNAs significantly less abundant (top, blue) or significantly more abundant (bottom, brown) in Grsf1KO relative to WT mouse muscle. (C) GO annotations of functional groups significantly different in Grsf1cKO muscles. Numbers in parentheses show fold-changes. (+), upregulated in Grsf1cKO vs WT; (-), downregulated in the Grsf1cKO vs WT. Brown: upregulated mRNAs confirmed by RT-qPCR analysis. Blue: downregulated mRNA confirmed by RT-qPCR analysis.

Figure 4. Microarray analysis of RNAs differentially expressed in skeletal muscle from Grsf1KO vs WT mice. (A) Volcano plot showing differentially expressed RNAs (including both mRNAs and long noncoding RNAs detected by probes in Agillent microarrays) in the Grsf1cKO vs WT RF muscles; n=3 mice for each genotype. (B) List of Top 25 RNAs significantly less abundant (top, blue) or significantly more abundant (bottom, brown) in Grsf1KO relative to WT mouse muscle. (C) GO annotations of functional groups significantly different in Grsf1cKO muscles. Numbers in parentheses show fold-changes. (+), upregulated in Grsf1cKO vs WT; (-), downregulated in the Grsf1cKO vs WT. Brown: upregulated mRNAs confirmed by RT-qPCR analysis. Blue: downregulated mRNA confirmed by RT-qPCR analysis.