Research Paper Volume 14, Issue 3 pp 1253—1264
Puerarin inhibits FUNDC1-mediated mitochondrial autophagy and CSE-induced apoptosis of human bronchial epithelial cells by activating the PI3K/AKT/mTOR signaling pathway
Figure 1. Puerarin promotes the proliferation and inhibits apoptosis of CSE-induced HBECs. HBECs was induced by 20% CSE and intervened with 50 μg/mL, 100 μg/mL and 200 μg/mL puerarin for 12 h, respectively. (A) MTT assay was used to measure the viability of CSE-induced HBECs. (B) Apoptosis of CSE-induced HBECs were detected by flow cytometry. (C) The protein expression of Cleaved caspase3 and Bax in CSE-induced HBECs were analyzed by Western blotting. β-actin was used as an invariant internal control for calculating protein-fold changes. N=6, * P<0.05, ** P<0.01.