Research Paper Volume 14, Issue 3 pp 1253—1264
Puerarin inhibits FUNDC1-mediated mitochondrial autophagy and CSE-induced apoptosis of human bronchial epithelial cells by activating the PI3K/AKT/mTOR signaling pathway
Figure 2. Puerarin inhibits mitochondrial autophagy of CSE-induced HBECs. HBECs was induced by 20% CSE and intervened with 50 μg/mL, 100 μg/mL and 200 μg/mL puerarin for 12 h, respectively. (A) Flow cytometry (JC-1) was used to detect changes in MMP level in CSE-induced HBECs. (B) Mitochondrial ROS levels were detected by flow cytometry (DCFH-DA). (C) The content of ATP in CSE-induced HBECs was detected with kits. (D) Western blotting was used to detect the expression of mitochondrial autophagy-related proteins such as PINK1 and Parkin. β-actin was used as the loading control. N=6, * P<0.05, ** P<0.01.