Research Paper Volume 14, Issue 3 pp 1253—1264
Puerarin inhibits FUNDC1-mediated mitochondrial autophagy and CSE-induced apoptosis of human bronchial epithelial cells by activating the PI3K/AKT/mTOR signaling pathway
Figure 3. Puerarin down-regulates the expression of DRP1 and FUNDC1. HBECs was induced by 20% CSE and intervened with 50 μg/mL, 100 μg/mL and 200 μg/mL puerarin for 12 h, respectively. (A) Relative mRNA expression of DRP1 was analyzed by RT-qPCR. (B) Western blotting was used to measure the protein expression of DRP1. (C) Relative expression of FUNDC1 was detected by RT-qPCR. (D) The protein expression of FUNDC1 and p-FUNDC1 in CSE-induced HBECs were analyzed by Western blotting. β-actin was used as an internal reference. N=6, * P<0.05, ** P<0.01.