Research Paper Volume 14, Issue 3 pp 1253—1264
Puerarin inhibits FUNDC1-mediated mitochondrial autophagy and CSE-induced apoptosis of human bronchial epithelial cells by activating the PI3K/AKT/mTOR signaling pathway
Figure 5. Puerarin inhibits apoptosis of CSE-induced HBECs by inhibiting mitochondrial autophagy. Mdivi, a mitochondrial autophagy inhibitor, treated 20% CSE-induced HBECs and then co-cultured with puerarin at a concentration of 200 μg/mL. (A) Western blotting was used to detect the expression of mitochondrial autophagy-related proteins such as PINK1 and Parkin. (B) MTT assay was used to measure the viability of CSE-induced HBECs. (C) Apoptosis of CSE-induced HBECs were detected by flow cytometry. (D) The protein expression of Cleaved caspase3 and Bax in CSE-induced HBECs were analyzed by Western blotting. β-actin was used as the loading control. N=6, ** P<0.01.