Figure 3. Panaxadiol induced apoptosis in PANC-1 and Patu8988 cells. (A–B) Flow cytometry of apoptosis demonstrated apoptosis ratio of pancreatic cancer cells was significantly increased after being incubated with different concentrations of panaxadiol, and apoptosis ratio = (Q2+Q3)/(Q1+Q2+Q3+Q4), *P < 0.05. (C) The expression of apoptosis-related proteins (Bcl-2, Bax, Cleaved caspase3) were detected by western blot. The ratios of Bcl-2/Bax of PANC-1 were 1.29, 0.64, 0.32 as the concentration of panaxadiol increased gradually. For Patu8988, the ratios were 4.04, 0.51, 0.33 accordingly. (D) qRT-PCR confirmed the same results as western blot from the expression of gene level. (E) Cleaved caspase3 was up-regulated in PANC-1 and Patu8988 cells after being treated with panaxadiol by performing immunofluorescence staining.