Research Paper Volume 13, Issue 19 pp 22843—22855

Allicin protects against myocardial I/R by accelerating angiogenesis via the miR-19a-3p/PI3K/AKT axis

Microarray analysis exposed the regulation of angiogenesis pathway, and gene miRNA-19a-3p might affect the Allicin-induced MI/R protection. (A) The volcano map showed the differently expressed miRNAs by the criteria of |fold change| >2 and P B–C) Functional classification of these differentially expressed genes based on the Gene Ontology (GO). (D) Partial results of the KEGG pathways. (E) The top 10 differentially expressed miRNAs in myocardium Model group compared with the Control group. (F) RT-PCR indicate that MI/R could induce the expression of miR-19a-3p, which can be further modulated by Allicin. (G) RT-PCR revealed that miR-19a-3p has downregulated in mice MI/R specimens compared with matched control tissues. (H) The Kaplan-Meier survival curve was performed to evaluate the diagnostic value of miR-19a- 3p in MI/R. (I) The potential binding site between miR-19a-3p and PIK3CA (PI3K) by Miranda. (J) The expression levels of miR-19a-3p were noticeably down-regulated in PI3K overexpressed macrophages.

Figure 4. Microarray analysis exposed the regulation of angiogenesis pathway, and gene miRNA-19a-3p might affect the Allicin-induced MI/R protection. (A) The volcano map showed the differently expressed miRNAs by the criteria of |fold change| >2 and P < 0.05 between MI/R and normal controls. (BC) Functional classification of these differentially expressed genes based on the Gene Ontology (GO). (D) Partial results of the KEGG pathways. (E) The top 10 differentially expressed miRNAs in myocardium Model group compared with the Control group. (F) RT-PCR indicate that MI/R could induce the expression of miR-19a-3p, which can be further modulated by Allicin. (G) RT-PCR revealed that miR-19a-3p has downregulated in mice MI/R specimens compared with matched control tissues. (H) The Kaplan-Meier survival curve was performed to evaluate the diagnostic value of miR-19a- 3p in MI/R. (I) The potential binding site between miR-19a-3p and PIK3CA (PI3K) by Miranda. (J) The expression levels of miR-19a-3p were noticeably down-regulated in PI3K overexpressed macrophages.