Research Paper Volume 13, Issue 19 pp 22963—22984

RUNX2 and LAMC2: promising pancreatic cancer biomarkers identified by an integrative data mining of pancreatic adenocarcinoma tissues


Figure 8. LAMC2 and RUNX2 regulate PC cell growth and migration through PI3K/AKT and MAPK pathway. (A, B) ASPC-1 cells were stably transfected with control shRNA (NC) or two shRNAs of different sequences targeting LAMC2 or RUNX2; cell samples were collected and subjected to Western blot analysis of p-AKT, p-c-Raf, p-MEK1/2, p-ERK1/2, p-P90RSK and p-MSK1. Actin was used as a loading control. The data expressed in right graphs represent the mean ± SEM (*p < 0.05).