Figure 3. Up-regulating MEG3 facilitated LPS-induced astrocyte inflammation. (A) After transfection of MEG3 overexpressing plasmids, the level of MEG3 in astrocytes was tested by RT-qPCR. (B–D) The contents of IL-1β (B), IL-6 (C), TNF-α (D) in astrocytes were monitored by ELISA after MEG3 up-regulation. (E–H) The levels of TLR4 (E), iNOS (F), COX2 (G), NF-κB (H), CXCL12 (I), CXCR4 (J), and Rac1 (K) in astrocytes after up-regulation of MEG3 were compared by WB. Data were expressed as mean±SD. n=3. **P<0.01, ***P<0.001 (vs. LPS group). &P<0.05, &&P<0.01 (vs. LPS+Vector group).