Research Paper Volume 13, Issue 19 pp 23004—23019

lncRNA MEG3 aggravated neuropathic pain and astrocyte overaction through mediating miR-130a-5p/CXCL12/CXCR4 axis

Up-regulating miR-130a-5p suppressed LPS-induced inflammation in astrocytes. (A) After transfection of miR-130a-5p mimics, the mRNA level of miR-130a-5p in astrocytes was tested by RT-qPCR. (B–D) The contents of IL-1β (B), IL-6 (C) and TNF-α (D) in astrocytes were compared by ELISA after up-regulation of miR-130a-5p. (E–H) WB detected the expression of TLR4 (E), iNOS (F), COX2(G), NF-κB (H), CXCL12 (I), CXCR4 (J), and Rac1 (K) in astrocytes after up-regulating miR-130a-5p. Data were expressed as mean±SD. n=3. **PPPP

Figure 4. Up-regulating miR-130a-5p suppressed LPS-induced inflammation in astrocytes. (A) After transfection of miR-130a-5p mimics, the mRNA level of miR-130a-5p in astrocytes was tested by RT-qPCR. (BD) The contents of IL-1β (B), IL-6 (C) and TNF-α (D) in astrocytes were compared by ELISA after up-regulation of miR-130a-5p. (EH) WB detected the expression of TLR4 (E), iNOS (F), COX2(G), NF-κB (H), CXCL12 (I), CXCR4 (J), and Rac1 (K) in astrocytes after up-regulating miR-130a-5p. Data were expressed as mean±SD. n=3. **P<0.01, ***P<0.001 (vs. LPS group). &P<0.05, &&P<0.01 (vs. LPS+miR-NC group).