Research Paper Volume 13, Issue 19 pp 23004—23019

lncRNA MEG3 aggravated neuropathic pain and astrocyte overaction through mediating miR-130a-5p/CXCL12/CXCR4 axis

Overexpressing miR-130a-5p reversed the MEG3-mediated astrocyte inflammation. MEG3 overexpression plasmids and miR-130a-5p mimics were transfected in LPS-induced astrocytes. (A, B) The mRNA profiles of MEG3 and miR-130a-5p in astrocytes were tested by RT-qPCR. (C) The protein levels of CXCL12, CXCR4, and Rac1 in astrocytes were examined by WB. D-F: The concentrations of IL-1β (D), IL-6 (E) and TNF-α (F) in astrocytes were determined by ELISA. (G) WB was utilized to test the expression of TLR4, iNOS, COX2 and NF-κB in astrocytes. Data were expressed as mean±SD. n=3. *PPPPPPPP

Figure 6. Overexpressing miR-130a-5p reversed the MEG3-mediated astrocyte inflammation. MEG3 overexpression plasmids and miR-130a-5p mimics were transfected in LPS-induced astrocytes. (A, B) The mRNA profiles of MEG3 and miR-130a-5p in astrocytes were tested by RT-qPCR. (C) The protein levels of CXCL12, CXCR4, and Rac1 in astrocytes were examined by WB. D-F: The concentrations of IL-1β (D), IL-6 (E) and TNF-α (F) in astrocytes were determined by ELISA. (G) WB was utilized to test the expression of TLR4, iNOS, COX2 and NF-κB in astrocytes. Data were expressed as mean±SD. n=3. *P<0.05, **P<0.01, ***P<0.001 (vs. Con group), #P<0.05, ##P<0.01 (vs. LPS group), &P<0.05, &&P<0.01, &&&P<0.001 (vs. LPS+MEG3 group).