Priority Research Paper Volume 13, Issue 19 pp 22629—22648

Green tea catechins EGCG and ECG enhance the fitness and lifespan of Caenorhabditis elegans by complex I inhibition

Increased lifespan, locomotion activity, and stress resistance after EGCG and ECG treatment. The representative outcome of lifespan assay of N2 wild-type nematodes in the presence of 2.5 μM EGCG versus DMSO. (A) The representative outcome of lifespan assay of N2 wild-type nematodes in the presence of 2.5 μM ECG versus control. (B) Locomotion quantification for N2 wild-type nematodes after 7 days exposure to DMSO, 2.5 μM EGCG, or 2.5 μM ECG. (C) The representative outcome of the survival analysis (h) of N2 nematodes in 50 mM paraquat solution after 7 days of pretreatment with EGCG (D) or ECG (E) in comparison to worms pretreated with DMSO. P-values are as indicated in the graphs. See Table 1 and Table 2 for corresponding detailed data and statistical analyses of lifespan assays and of paraquat stress assay, respectively.

Figure 1. Increased lifespan, locomotion activity, and stress resistance after EGCG and ECG treatment. The representative outcome of lifespan assay of N2 wild-type nematodes in the presence of 2.5 μM EGCG versus DMSO. (A) The representative outcome of lifespan assay of N2 wild-type nematodes in the presence of 2.5 μM ECG versus control. (B) Locomotion quantification for N2 wild-type nematodes after 7 days exposure to DMSO, 2.5 μM EGCG, or 2.5 μM ECG. (C) The representative outcome of the survival analysis (h) of N2 nematodes in 50 mM paraquat solution after 7 days of pretreatment with EGCG (D) or ECG (E) in comparison to worms pretreated with DMSO. P-values are as indicated in the graphs. See Table 1 and Table 2 for corresponding detailed data and statistical analyses of lifespan assays and of paraquat stress assay, respectively.