Priority Research Paper Volume 13, Issue 19 pp 22629—22648

Green tea catechins EGCG and ECG enhance the fitness and lifespan of Caenorhabditis elegans by complex I inhibition

EGCG and ECG induce a drop in cellular ATP levels and require AMPK/SIRT1 signaling to extend lifespan.14CO2 production of N2 wild-type nematodes after treatment with 0.1% DMSO, 2.5 μM EGCG or 2.5 μM ECG for the indicated time. (A) ATP content for various incubation periods of N2 wild-type nematodes with 0.1% DMSO or 2.5 μM EGCG. (B) ATP content for different incubation periods of N2 wild-type nematodes with 0.1% DMSO or 2.5 μM EGCG. (C) The representative outcome of lifespan assay of aak-2 mutants treated with 0.1% DMSO versus 2.5 μM EGCG (D) or 2.5 μM ECG. (E) The representative outcome of lifespan assay of sir-2.1 mutants treated with 0.1% DMSO versus 2.5 μM EGCG (F) or 2.5 μM ECG. (G) P-values are as indicated in the graphs. See Table 1 for corresponding detailed data and statistical analyses of lifespan assays.

Figure 3. EGCG and ECG induce a drop in cellular ATP levels and require AMPK/SIRT1 signaling to extend lifespan.14CO2 production of N2 wild-type nematodes after treatment with 0.1% DMSO, 2.5 μM EGCG or 2.5 μM ECG for the indicated time. (A) ATP content for various incubation periods of N2 wild-type nematodes with 0.1% DMSO or 2.5 μM EGCG. (B) ATP content for different incubation periods of N2 wild-type nematodes with 0.1% DMSO or 2.5 μM EGCG. (C) The representative outcome of lifespan assay of aak-2 mutants treated with 0.1% DMSO versus 2.5 μM EGCG (D) or 2.5 μM ECG. (E) The representative outcome of lifespan assay of sir-2.1 mutants treated with 0.1% DMSO versus 2.5 μM EGCG (F) or 2.5 μM ECG. (G) P-values are as indicated in the graphs. See Table 1 for corresponding detailed data and statistical analyses of lifespan assays.