Research Paper Volume 13, Issue 19 pp 23096—23107

Zhoushi Qi Ling decoction represses docetaxel resistance and glycolysis of castration-resistant prostate cancer via regulation of SNHG10/miR-1271-5p/TRIM66 axis

Qi Ling impaired docetaxel resistance of CRPC cells. (A and B) Parental PC3 cells and the DOC-resistant counterpart (PC3-DR) were subjected to indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (C and D) Parental DU145 cells and the DOC-resistant counterpart (DU145-DR) were subjected to the indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (E and F) PC3-DR cells was cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of the indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (G and H) DU145-DR cells was cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of the indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (I) Colony formation assay showed cell viabilities of PC3-DR and DU145-DR cells cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of docetaxel (10 nM). (J and K) Cell apoptosis of PC3-DR and DU145-DR cells cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of docetaxel (10 nM) was measured by DNA fragmentation and Caspase-3 activity assays. Values are mean ± SD. **P ***P

Figure 1. Qi Ling impaired docetaxel resistance of CRPC cells. (A and B) Parental PC3 cells and the DOC-resistant counterpart (PC3-DR) were subjected to indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (C and D) Parental DU145 cells and the DOC-resistant counterpart (DU145-DR) were subjected to the indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (E and F) PC3-DR cells was cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of the indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (G and H) DU145-DR cells was cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of the indicated concentrations of docetaxel (0–400 nM) for 48 h, cell viability and IC50 value of docetaxel were determined by CCK-8 assay. (I) Colony formation assay showed cell viabilities of PC3-DR and DU145-DR cells cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of docetaxel (10 nM). (J and K) Cell apoptosis of PC3-DR and DU145-DR cells cultured in normal media (ctrl) or media supplement with Qi Ling (QL-treatment) with the treatment of docetaxel (10 nM) was measured by DNA fragmentation and Caspase-3 activity assays. Values are mean ± SD. **P < 0.01; ***P < 0.001.