Research Paper Volume 13, Issue 19 pp 23096—23107

Zhoushi Qi Ling decoction represses docetaxel resistance and glycolysis of castration-resistant prostate cancer via regulation of SNHG10/miR-1271-5p/TRIM66 axis

SNHG10 up-regulated TRIM66 via sponging miR-1271-5p. (A) miR-1271-5p binding sites in SNHG10 and site mutagenesis. (B and C) Luciferase activity in PC3-DR and DU145-DR cells. (D and E) RNA levels of SNHG10 and miR-1271-5p in PC3-DR and DU145-DR cells transfected with siRNAs against SNHG10 (si-SNHG10-1 and si-SNHG10-2) or negative control siRNAs (si-NC) were detected by qRT-PCR. (F) miR-1271-5p binding sites in TRIM66 3’UTR and site mutagenesis. (G and H) Relative luciferase activity in PC3-DR and DU145-DR cells. (I and J) mRNA and protein level of TRIM66 in PC3-DR and DU145-DR cells transfected with miR-1271-5p mimics or mimics negative control (miR-NC) were determined by qRT-PCR and Western blot. (K and L) mRNA and protein level of TRIM66 in PC3-DR and DU145-DR cells transfected with siRNAs against SNHG10 (si-SNHG10-1 and si-SNHG10-2) or negative control siRNAs (si-NC) were determined by qRT-PCR and Western blot. Values are mean ± SD. *P **P

Figure 4. SNHG10 up-regulated TRIM66 via sponging miR-1271-5p. (A) miR-1271-5p binding sites in SNHG10 and site mutagenesis. (B and C) Luciferase activity in PC3-DR and DU145-DR cells. (D and E) RNA levels of SNHG10 and miR-1271-5p in PC3-DR and DU145-DR cells transfected with siRNAs against SNHG10 (si-SNHG10-1 and si-SNHG10-2) or negative control siRNAs (si-NC) were detected by qRT-PCR. (F) miR-1271-5p binding sites in TRIM66 3’UTR and site mutagenesis. (G and H) Relative luciferase activity in PC3-DR and DU145-DR cells. (I and J) mRNA and protein level of TRIM66 in PC3-DR and DU145-DR cells transfected with miR-1271-5p mimics or mimics negative control (miR-NC) were determined by qRT-PCR and Western blot. (K and L) mRNA and protein level of TRIM66 in PC3-DR and DU145-DR cells transfected with siRNAs against SNHG10 (si-SNHG10-1 and si-SNHG10-2) or negative control siRNAs (si-NC) were determined by qRT-PCR and Western blot. Values are mean ± SD. *P < 0.05; **P < 0.01.