**Figure 2.** **Silencing of lncRNA-SNHG1 inhibited M2 macrophage polarization.** (**A**, **B**) Two siRNAs of lncRNA-SNHG1 were construct and knockdown efficiency was confirmed in RAW264.7 cells and BMDMs using qRT-PCR analysis. Two-tailed t-test was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (**C**, **D**) Flow-cytometric analysis was performed to analyze the percentage of CD206^{+} cells. RAW264.7 cells were transfected with two siRNAs of lncRNA-SNHG1 and negative control siRNA for 48h and treated with IL4/IL13 or LPS/INFγ for 72h. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (**E**, **F**) Flow-cytometric analysis was performed to analyze the percentage of F4/80^{+}CD206^{+} cells. BMDMs were transfected with two siRNAs of lncRNA-SNHG1 or negative control siRNA for 48h and treated with IL4/IL13 or LPS/INFγ for 72h. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (**G**) mRNA of M2 polarization marker genes in RAW264.7 cells was quantified by qRT-PCR analysis. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (**H**) Overexpression of lncRNA-SNHG1 was identified via performing qRT-PCR analysis. pcDNA3.1 empty vector acted as a negative control. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (**I**, **J**) Flow-cytometric analysis was performed to analyze the percentage of CD206^{+} cells. RAW264.7 cells were transfected with expression plasmid of lncRNA-SNHG1 or pcDNA3.1 and treated with IL4/IL13 or LPS/INFγ for 72h. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01.