Research Paper Volume 13, Issue 19 pp 23169—23181

LncRNA-SNHG1 promotes macrophage M2-like polarization and contributes to breast cancer growth and metastasis

Silencing of lncRNA-SNHG1 in RAW264.7 cells inhibited tumor growth and angiogenesis. (A) qRT-PCR analysis was performed to measure the RNA level of lncRNA-SNHG1 after transfection of Adv-lncRNA-SNHG1 in RAW264.7 cells. Two-tailed t-test was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **PB, C) The tumors formed in mice at the endpoint of mice study. Two-tailed t-test was used for the statistical analysis. n=7 mice per group. The bar indicates the SD values. **PD) Body weight of mice was measured at the endpoint of mice study. Two-tailed t-test was used for the statistical analysis. n=7 mice per group. The bar indicates the SD values. **PE) Survival curve was shown by counting dead mice of three groups. n=20 mice per group. #PF) IHC staining of CD206 of tumor tissues was performed. Two-tailed t-test was used for the statistical analysis. n=5 mice per group. The bar indicates the SD values. **PG) qRT-PCR analysis was performed to measure the RNA level of M2 polarization markers in tumor tissues. n=5 mice per group. The bar indicates the SD values. **PH) IHC staining of CD31 of tumor tissues was performed. Two-tailed t-test was used for the statistical analysis. n=5 mice per group. The bar indicates the SD values. **P

Figure 5. Silencing of lncRNA-SNHG1 in RAW264.7 cells inhibited tumor growth and angiogenesis. (A) qRT-PCR analysis was performed to measure the RNA level of lncRNA-SNHG1 after transfection of Adv-lncRNA-SNHG1 in RAW264.7 cells. Two-tailed t-test was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (B, C) The tumors formed in mice at the endpoint of mice study. Two-tailed t-test was used for the statistical analysis. n=7 mice per group. The bar indicates the SD values. **P<0.01. (D) Body weight of mice was measured at the endpoint of mice study. Two-tailed t-test was used for the statistical analysis. n=7 mice per group. The bar indicates the SD values. **P<0.01. (E) Survival curve was shown by counting dead mice of three groups. n=20 mice per group. #P<0.05 vs. sh-1 group, *P<0.05 vs. sh-2 group. (F) IHC staining of CD206 of tumor tissues was performed. Two-tailed t-test was used for the statistical analysis. n=5 mice per group. The bar indicates the SD values. **P<0.01. (G) qRT-PCR analysis was performed to measure the RNA level of M2 polarization markers in tumor tissues. n=5 mice per group. The bar indicates the SD values. **P<0.01. (H) IHC staining of CD31 of tumor tissues was performed. Two-tailed t-test was used for the statistical analysis. n=5 mice per group. The bar indicates the SD values. **P<0.01.