Research Paper Volume 13, Issue 19 pp 23193—23209

Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome

Systematic understanding of Schisandrin B induced AMPK/Nrf2 signaling pathway in vivo and vitro model of colitis. (A) Network of potential Schisandrin B targets; (B, C) p-AMPK and Nrf2 protein expression in mouse colon tissue; (D, E) p-AMPK and Nrf2 protein expression in intestinal epithelial cells induced by LPS + ATP. ##P

Figure 4. Systematic understanding of Schisandrin B induced AMPK/Nrf2 signaling pathway in vivo and vitro model of colitis. (A) Network of potential Schisandrin B targets; (B, C) p-AMPK and Nrf2 protein expression in mouse colon tissue; (D, E) p-AMPK and Nrf2 protein expression in intestinal epithelial cells induced by LPS + ATP. ##P<0.01 vs control group; **P<0.01 vs DSS- induced colitis group. Control: blank control group; Colitis: DSS- induced colitis group; Colitis+SCH: DSS- induced colitis mice with Schisandrin. ##P<0.01 vs MDSO group; **P<0.01 vs LPS+ATP induced intestinal epithelial cells group. MDSO: blank control group; LPS+ATP: intestinal epithelial cells with LPS+ATP group; LPS+ATP +SCH: intestinal epithelial cells induced by LPS+ATP with Schisandrin. SCH, Schisandrin B. Data were expressed as mean ± SEM.