Research Paper Volume 13, Issue 19 pp 23193—23209

Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome

Schisandrin B reduced ROS-induced mitochondrial damage. (A) Methods: the total number of JC-1 in intestinal epithelial cells induced by LPS + ATP; (B) the level of ROS in intestinal epithelial cells induced by LPS + ATP; (C) mitochondria and ROS in intestinal epithelial cells by immunofluorescence; (D) mitochondria in intestinal epithelial cells by electron microscope. ##P$$P

Figure 8. Schisandrin B reduced ROS-induced mitochondrial damage. (A) Methods: the total number of JC-1 in intestinal epithelial cells induced by LPS + ATP; (B) the level of ROS in intestinal epithelial cells induced by LPS + ATP; (C) mitochondria and ROS in intestinal epithelial cells by immunofluorescence; (D) mitochondria in intestinal epithelial cells by electron microscope. ##P<0.01 vs MDSO group; **P<0.01 vs LPS+ATP induced intestinal epithelial cells group; $$P<0.01 vs LPS+ATP+SCH group. MDSO: blank control group; LPS+ATP: intestinal epithelial cells with LPS+ATP group; LPS+ATP +SCH: intestinal epithelial cells induced by LPS+ATP with Schisandrin; LPS+ATP+SCH+AMPK i: intestinal epithelial cells induced by LPS+ATP, Schisandrin and AMPK inhibitor. SCH, Schisandrin B. Data were expressed as mean ± SEM.