Research Paper Volume 13, Issue 19 pp 22710—22731

Neurovascular dysfunction and neuroinflammation in a Cockayne syndrome mouse model

CX mice display astrocyte and microglia activation in the brain. (A) Reactive astrocytes number increase in CX brain cortex and corpus callosum, as measured by GFAP staining. (B) C3, a marker of active astrocytes co-stains with GFAP in CX mice. (C) Microglia number in CX brain cortex and corpus callosum, shown by Iba1 positive cells. (D) CD68, a marker or active microglia co-stains with Iba1 in CX mice brain, n = 4 for all experiments. Data are presented as mean ± SE. Two way Anova followed by Tukey’s post hoc test, n = 4. P values of less than or equal to 0.05, 0.01, 0.001 and 0.0001 are indicated by asterisks (*) when comparing within the same age group and by the pound sign (#) when comparing between age groups. Abbreviations: Ctrl: control; Wk: weeks.

Figure 5. CX mice display astrocyte and microglia activation in the brain. (A) Reactive astrocytes number increase in CX brain cortex and corpus callosum, as measured by GFAP staining. (B) C3, a marker of active astrocytes co-stains with GFAP in CX mice. (C) Microglia number in CX brain cortex and corpus callosum, shown by Iba1 positive cells. (D) CD68, a marker or active microglia co-stains with Iba1 in CX mice brain, n = 4 for all experiments. Data are presented as mean ± SE. Two way Anova followed by Tukey’s post hoc test, n = 4. P values of less than or equal to 0.05, 0.01, 0.001 and 0.0001 are indicated by asterisks (*) when comparing within the same age group and by the pound sign (#) when comparing between age groups. Abbreviations: Ctrl: control; Wk: weeks.