Research Paper Volume 13, Issue 19 pp 23361—23375

piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification

BMP2 overexpression reversed the effect of METTL3 silence on osteogenic differentiation. BMSCs were infected with Lv-sh-METTL3 alone or together with Lv-BMP2, and then cultured in osteogenic differentiation medium for 14 days. (A, B) The protein level of METTL3 and BMP2 were detected on day 14. (C, D) The protein expression of METTL3, RUNX2, COL1A1, OCN and OPN were assessed on day 14. (E) Images of ALP staining (100×). (F) ALP activity was measured on day 14. (G) Images of ARS staining (100×). (H) Quantitative analysis of ARS accumulation on day 14. N = 5 in each group. *P

Figure 6. BMP2 overexpression reversed the effect of METTL3 silence on osteogenic differentiation. BMSCs were infected with Lv-sh-METTL3 alone or together with Lv-BMP2, and then cultured in osteogenic differentiation medium for 14 days. (A, B) The protein level of METTL3 and BMP2 were detected on day 14. (C, D) The protein expression of METTL3, RUNX2, COL1A1, OCN and OPN were assessed on day 14. (E) Images of ALP staining (100×). (F) ALP activity was measured on day 14. (G) Images of ARS staining (100×). (H) Quantitative analysis of ARS accumulation on day 14. N = 5 in each group. *P < 0.05. Each test was independently repeated at least three times.