Research Paper Advance Articles

Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner

Over-expression of Nmnat2 suppresses Aβ productions and up-regulates ADAM10 in N2a/APPswe cells. N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or the empty vector for forty eight hours, and then mRNA level of ADAM10 and protein levels of ADAM10, α-CTF, and Aβ were detected by real-time PCR (A), western blot (B, D, E), and quantitative analysis (C, F). The data were representative of at least three independent experiments and expressed as means ± S.D.. *P P

Figure 2. Over-expression of Nmnat2 suppresses Aβ productions and up-regulates ADAM10 in N2a/APPswe cells. N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or the empty vector for forty eight hours, and then mRNA level of ADAM10 and protein levels of ADAM10, α-CTF, and Aβ were detected by real-time PCR (A), western blot (B, D, E), and quantitative analysis (C, F). The data were representative of at least three independent experiments and expressed as means ± S.D.. *P < 0.05, **P < 0.01 vs control.