Research Paper Advance Articles

Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner

Nmnat2 up-regulates the ratio of NAD+/NADH in vitro and in vivo. (A) The cortex extracts were prepared from 10 months old Tg2576 mice (Tg) and the age-matched littermates (Non-Tg), then the ratio of NAD+/NADH was measured by NAD+/NADH quantification kit (A) (n=5 for each group). The data were expressed as means ± S.D. **P B) N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or the empty vector for forty eight hours, and then the ratio of NAD+/NADH was detected by NAD+/NADH quantification kit (B). The data were representative from three independent experiments at least and expressed as means ± S.D.. **P

Figure 6. Nmnat2 up-regulates the ratio of NAD+/NADH in vitro and in vivo. (A) The cortex extracts were prepared from 10 months old Tg2576 mice (Tg) and the age-matched littermates (Non-Tg), then the ratio of NAD+/NADH was measured by NAD+/NADH quantification kit (A) (n=5 for each group). The data were expressed as means ± S.D. **P < 0.01 vs control. (B) N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or the empty vector for forty eight hours, and then the ratio of NAD+/NADH was detected by NAD+/NADH quantification kit (B). The data were representative from three independent experiments at least and expressed as means ± S.D.. **P < 0.01 vs control.