Research Paper Volume 13, Issue 20 pp 23652—23671

Up-regulation of SIRT1 induced by 17beta-estradiol promotes autophagy and inhibits apoptosis in osteoblasts

Effect of 17β-E2 on the expression level of LC3 in hFOB1.19 osteoblasts. (A) Western blotting showed the expression of LC3 protein in osteoblasts treated with 17β-E2 (0, 10-8, 10-7, 10-6 M) for 24 h. (B) Western blotting showed the expression of LC3 in osteoblasts treated with 10-6 M 17β-E2 for different time (0, 2, 4, 24, 36 h). The histograms showed the relative expression level of LC3 normalized to β-actin, and the densitometry analysis of the blots were estimated by using the Image J Software. The data were expressed as the means ± SD from 3 independent experiments. Statistically significance was evaluated using the Student’s t-test. * P

Figure 2. Effect of 17β-E2 on the expression level of LC3 in hFOB1.19 osteoblasts. (A) Western blotting showed the expression of LC3 protein in osteoblasts treated with 17β-E2 (0, 10-8, 10-7, 10-6 M) for 24 h. (B) Western blotting showed the expression of LC3 in osteoblasts treated with 10-6 M 17β-E2 for different time (0, 2, 4, 24, 36 h). The histograms showed the relative expression level of LC3 normalized to β-actin, and the densitometry analysis of the blots were estimated by using the Image J Software. The data were expressed as the means ± SD from 3 independent experiments. Statistically significance was evaluated using the Student’s t-test. * P < 0.05 vs. the control group was considered significant.