Research Paper Volume 13, Issue 20 pp 23652—23671

Up-regulation of SIRT1 induced by 17beta-estradiol promotes autophagy and inhibits apoptosis in osteoblasts


Figure 4. Effect of SIRT1 up-regulation induced by17β-E2 on the expression of LC3 in hFOB1.19 osteoblasts. SRT1720 or EX527 regulated the expression of LC3 in 17β-E2-treated osteoblasts, as determined using immunofluorescence staining. Nuclei were stained with DAPI (blue). Representative images and quantified analysis of immunofluorescence staining showing the LC3 in cells. White bar = 50 μm. The histogram showed the number of LC3 puncta in various groups as indicated. The data were expressed as the means ± SD from 3 independent experiments. Statistically significance was evaluated using the Student’s t-test. * P < 0.05 vs. the control group was considered significant.